Uji Efektivitas Antibakteri Larutan Lo’i Keta dan Ekstrak Methanol Lo’i Keta Terhadap Pertumbuhan Staphyloccus Epidermidis dan Pseudomonas Aeruginosa
DOI:
https://doi.org/10.33394/bioscientist.v13i3.14496Keywords:
Antibakteri, Lo'i Keta, Staphylococcus epidermidis, Pseudomonas aeruginosa, SumuranAbstract
This study aimed to determine the antibacterial activity of Lo’i keta solution and methanol extract against the growth of Staphylococcus epidermidis and Pseudomonas aeruginosa. The research employed a true experimental design using a Posttest Only Control Group Design with the well diffusion method. A total of 80 samples were divided into 20 treatment groups with concentrations of 25%, 50%, 75%, and 100%, along with a positive control (ciprofloxacin) and a negative control (distilled water). GC-MS analysis revealed nine bioactive compounds in the methanol extract of Lo’i keta. The results showed that the Lo’i keta solution inhibited the growth of Staphylococcus epidermidis with inhibition zones ranging from 15–24 mm, while the methanol extract produced larger inhibition zones of 18–32 mm. Against Pseudomonas aeruginosa, only the methanol extract demonstrated antibacterial activity with inhibition zones of 13–17 mm, whereas the solution showed no inhibitory effect. The Kruskal-Wallis test indicated significant differences among the treatment groups. In conclusion, both the Lo’i keta solution and the methanol extract were effective in inhibiting the growth of Staphylococcus epidermidis, while antibacterial activity against Pseudomonas aeruginosa was observed only in the methanol extract. Lo’i keta demonstrates potential to be developed as a traditional medicine-based antibacterial alternative.
References
Aditiya, A. S. D. (2021). Uji Efektivitas Sediaan Krim Ekstrak Bunga Melati (Jasminum Sambac L.) Terhadap Pertumbuhan Bakteri Propionibacterium Acne. Jurnal Farmasi Dan Kesehatan Indonesia, 1(2), 1–12. https://doi.org/10.61179/jfki.v1i2.234
Azis, A., Izzati, M., Biologi, S. H.-J. A., & 2015, U. (2015). Aktivitas antioksidan dan nilai gizi dari beberapa jenis beras dan millet sebagai bahan pangan fungsional Indonesia. Jurnal Biologi, 4(1), 45–61.
Daud, N. S., Arni, D. P., Idris, S. A., & Saehu, M. S. (2023). Uji Aktivitas Antibakteri Ekstrak Batang Meistera chinensis Terhadap Escherichia coli ATCC 35218. Warta Farmasi, 12(1), 8-18.
Desbois, A. P., & Smith, V. J. (2010). Antibacterial free fatty acids: Activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology, 85(6), 1629–1642. https://doi.org/10.1007/s00253-009-2355-3
Fadia, Nurlailah, Helmiah, T. E., & Lutpiatina, L. (2020). Efektivitas Ekstrak Etanol Daun Kirinyuh (Chromolaena Odorata L) Sebagai Antibakteri Salmonella Typhi Dan Staphylococcus Aureus. In Jurnal Riset Kefarmasian Indonesia (Vol. 2, Issue 3). https://doi.org/10.33759/jrki.v2i3.104
Fitriyah, D., Putri Ayu, D., Dewi Puspita, S., Kartika, R. C., & Ubaidillah, M. (2022). Kandungan Nutrisi dan Aktivitas Antimikroba Ekstrak Beras Merah Nutrient Content and Antimicrobial Activity of Red Rice Extract. Jurnal Pangan Dan Gizi , 12(2), 30–36.
Gao, J., Liang, L., Zhu, Y., Qiu, S., Wang, T., & Zhang, L. (2016). Ligand and structure-based approaches for the identification of peptide deformylase inhibitors as antibacterial drugs. International Journal of Molecular Sciences, 17(7). https://doi.org/10.3390/ijms17071141
Holert, J., Brown, K., Hashimi, A., Eltis, L. D., & Mohn, W. W. (2020). Steryl ester formation and accumulation in steroid-degrading bacteria. Applied and Environmental Microbiology, 86(2). https://doi.org/10.1128/AEM.02353-19
Julianto, T. S. (2019). Fitokimia Tinjauan Metabolit Sekunder dan Skrining fitokimia. In Jakarta penerbit buku kedokteran EGC (Vol. 53, Issue 9).
Kaur, G., Kapoor, S., Kaundal, S., Dutta, D., & Thakur, K. G. (2020). Structure-Guided Designing and Evaluation of Peptides Targeting Bacterial Transcription. Frontiers in Bioengineering and Biotechnology, 8(September), 1–10. https://doi.org/10.3389/fbioe.2020.00797
Lidjaja, L. N. (2022). Karakteristik Penyakit Infeksi Kulit di Poliklinik Klinik Pratama Panti Siwi Jember, Januari 2018–Desember 2020. Cermin Dunia Kedokteran, 49(8), 423–426. https://doi.org/10.55175/cdk.v49i8.266
Maharani, D., Rafika, Hasan, Z. A., & Artati. (2023). Pengaruh Replikasi Pemanasan Media Nutrient Agar Terhadap Nutrisi Medua, pH Media Dan Jumlah Koloni Bakteri. Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia, 2(1), 73–85.
Naid, T., & Nuryanti, S. (2024). Aktivitas Antibakteri Ekstrak Metanol Daun Bidara (Ziziphus mauritiana Lam) Terhadap Bakteri Uji Penyebab Luka Infeksi Dengan Metode Difusi Agar. Makassar Pharmaceutical Science Journal, 2(2), 2024–2182. https://journal.farmasi.umi.ac.id/index.php/mpsj
Namvar, A. E., Bastarahang, S., Abbasi, N., Ghehi, G. S., Farhadbakhtiarian, S., Arezi, P., Hosseini, M., Baravati, S. Z., Jokar, Z., & Chermahin, S. G. (2014). Clinical characteristics of Staphylococcus epidermidis: a systematic review. GMS Hygiene and Infection Control, 9(3), Doc23. https://doi.org/10.3205/dgkh000243
Nikmah, I. S. (2022). Uji Antibakteri, Formulasi dan Uji Fisik Ekstrak Terpurifikasi Daun Katuk (Sauropus androgynus) terhadap Pseudomonas aeruginosa ATCC 27853. Skripsi, 1–88.
Pang, Z., Raudonis, R., Glick, B. R., Lin, T. J., & Cheng, Z. (2019). Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and alternative therapeutic strategies. Biotechnology Advances, 37(1), 177–192. https://doi.org/10.1016/j.biotechadv.2018.11.013
Putri, C. I., Wardhana, M. F., & Andrifianie, F. (2023). Literature Review: Kejadian Resistensi Pada Penggunaan Antibiotik. Muhammad Iqbal | Literature Review: Kejadian Resistensi Pada Penggunaan Antibiotik Medula |, 13, 219.
Rahayu, N. S., Puteri, A. D., & Isnaeni, L. M. A. (2023). Hubungan Perilaku Masyarakat Dan Penggunaan Air Sungai Dengan Gangguan Penyakit Kulit Di Desa Kampung Pinang Wilayah Kerja Puskesmas Pantai Raja. Jurnal Imliah Ilmu Kesehatan, 1(3), 2023.
Robertus, T. (2024). Mekanisme Resistensi Pseudomonas Aeruginosa Terhadap Antibiotik. Jurnal Penelitian Dan Karya Ilmiah Lembaga Penelitian Universitas Trisakti, 9, 214–221. https://doi.org/10.25105/pdk.v9i1.18185
Rostikawati, R. T., & Supratman, L. (2021). Uji Antibakteri Obat Kumur Ekstrak Etanol Tanaman Ciplukan (Physalis angulata l.) terhadap Bakteri Gram Positif. Quagga: Jurnal Pendidikan dan Biologi 13(1): 103-107.
Sriyono, R. A. N., & Andriani, I. (2013). Antibacterial power Ethanol Extract Skin Mangosteen (Garcinia Mangostana Linn.) Against bacteria Porphyromonas gingivalis. Idj, 2(2), 76–82.
Sugiaman VK, Djuanda R, Naliani S, Alfiyola E, Winardi J, and Demolsky WL. Antibacterial Differences Effect between the Onion Extract (Allium cepa L.) and Lemon Juice (Citrus limon (L.) Burm.f.) on in vitro Growth of Enterococcus faecalis. J of International Dental and Medical Research. 2023; 16(1): 111-116.
Wijayanti, T., & Setiawan, D. C. (2018). Eksplorasi Senyawa Metabolit Sekunder Pada Kulit Batang Tanaman Duwet (Syzygium Cumini L.) Dengan Metode Liquid Chromatograph Mass Spectrometry (LCMS). Bioma :Jurnal Ilmiah Biologi, 7(2), 196–210. https://doi.org/10.26877/bioma.v7i2.2757
Zein. Rifdah. (2022). Aktivitas Antibakteri Ekstrak Daun Delima (Punica Granatum L.) Dan Keragaman Genetik Berdasarkan Marka Random Amplified Polymorphic Dna. Program Pascasarjana Universitas Hasanuddin Makassar
Zabir, R. A. (2018). Uji Aktivitas Penghambatan Ekstrak Etanol Daun Delima (Punicagranatum L.) Terhadap Bakteri Mycobacterium tuberculosis. In Photosynthetica, 2(1).
Zhang, S., Xiong, J., Lou, W., Ning, Z., Zhang, D., & Yang, J. (2018). The identification of critical lethal action in antimicrobial mechanism of glycerol monomyristate against foodborne pathogens. BioRxiv. https://doi.org/10.1101/336354
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Dilfa Atri Safari
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
