Pengaruh Pemberian Ekstrak Etanol Buah Gandaria (Bouea macrophylla Griff.) Terhadap Profil Lipid Tikus Putih (Rattus norvegicus L.) Yang Diinduksi Karbon Tetraklorida (CCI4)
DOI:
https://doi.org/10.33394/bioscientist.v13i3.17449Keywords:
Buah gandaria; profil lipid; karbon tetraklorida (CCl4); tikus putih (Rattus norvegicus L.)Abstract
This study aimed to evaluate the effect of ethanolic extract of gandaria fruit (Bouea macrophylla Griff.) on the lipid profile of male white rats (Rattus norvegicus L.) induced with carbon tetrachloride (CCl₄). The study highlights the potential hypolipidemic activity of the extract by measuring levels of total cholesterol, triglycerides, Low Density Lipoprotein (LDL), and High Density Lipoprotein (HDL). A Completely Randomized Design (CRD) was employed, involving 25 rats divided into five groups: negative control, positive control (CCl₄ without treatment), and three treatment groups administered the extract at doses of 100, 150, and 200 mg/kg body weight for 30 days. Data were analyzed using ANOVA followed by Duncan’s Multiple Range Test (DMRT) at a significance level of p < 0.05. The results showed that the 200 mg/kg dose significantly reduced levels of total cholesterol, triglycerides, and LDL, while the highest increase in HDL was observed at the 100 mg/kg dose. These findings also indicate that the ethanolic extract of Bouea macrophylla fruit exhibits dose-dependent hypolipidemic and antioxidant effects and has potential as a natural therapeutic candidate for improving lipid profile disorders caused by chemically induced liver damage.
References
Amalia, R. N., Husnarika, F., & Syukriah. (2023). Pengaruh pemberian ekstrak etanol daun asam jawa (Tamarindus indica L.) terhadap profil lipid tikus putih (Rattus norvegicus) yang diinduksi minyak jelantah. Biologi Education Science & Technology, 6(1), 515–521.
Angela, J., & Sumbayak, E. M. (2017). Gambaran testis mencit yang diinduksi karbon tetraklorida dan diberi infusa umbi bawang dayak (Eleutherine palmifolia). Jurnal Kedokteran Meditek, 23(62), 11–20.
Arisandi, S., Hermawan, D., & Muhani, N. (2022). Durasi tidur dan kadar kolesterol total sebagai faktor risiko kejadian obesitas pada remaja akhir. Jurnal Medika Malahayati, 6(1), 257–264.
Boots, A. W., Haenen, G. R., & Bast, A. (2008). Health effects of quercetin: From antioxidant to nutraceutical. European Journal of Pharmacology, 585(2–3), 325–337.
Domínguez, R., Pateiro, M., Gagaoua, M., Barba, F. J., Zhang, W., & Lorenzo, J. M. (2019). A comprehensive review on lipid oxidation in meat and meat products. Antioxidants, 8(10), 429.
Dwitiyanti, H., Sunaryo, I., & Resty, K. (2016). Uji aktivitas antihiperkolesterolemia fraksi etil asetat ekstrak daun kelor (Moringa oleifera Lam.) terhadap kadar kolesterol total dan LDL kolesterol pada hamster hiperkolesterolemia. Jurnal Pharmacy, 12(2), 153–163.
Elsawy, H., Gehan, M. B., Azza, S., Basem, M. A., Abdullah, M. A., & Ashraf, M. A. (2019). Rutin improves hepatorenal toxicity and hypogonadism caused by carbon tetrachloride (CCl₄). PeerJ, 7, 7011.
Elgawish, M. H., & Zakaria, M. A. (2015). The effectiveness of intensive versus standard physical therapy for motor progress in children with spastic cerebral palsy. Egyptian Rheumatology and Rehabilitation, 42(1), 1–6.
Fu’adah, N., et al. (2022). Kandungan senyawa antioksidan alami pada buah gandaria dan potensinya terhadap penyakit kronis. Jurnal Gizi dan Tanaman Obat, 4(1), 50–56.
Galati, G., & O'Brien, P. J. (2004). Potential toxicity of flavonoids and other dietary phenolics: Significance for their chemopreventive and anticancer properties. Free Radical Biology and Medicine, 37(3), 287–303.
Isdadiyanto, S., Sitasiwi, A. J., & Mardiati, S. M. (2024). Profil Lipid Tikus (Rattus norvegicus L.) Hiperlipidemia Setelah Terpapar Ekstrak Etanol Biji Mahoni (Swietenia mahagoni). Buletin Anatomi Dan Fisiologi, 9(1), 85–92.
Li, J., et al. (2019). Flavonoids promote ABCA1 expression and cholesterol efflux through LXRα activation in macrophages. Journal of Lipid Research, 60(3), 566–578.
Lau, S. H. A., Sartini, S., & Lallo, S. (2019). Potensi Antioksidan Ekstrak Etanol Daun Sambung Nyawa (Gynura procumbens) Terenkapsulasi Maltodextrin Dan Pengaruhnya Terhadap Kadar MDA Darah Tikus Wistar (Rattus novergicus) Jantan Yang Diinduksi CCl4. Majalah Farmasi Dan Farmakologi, 22(3), 93–98.
Maurito, N. F., Husnarika, F., & Syukriah. (2024). Uji aktivitas antidiabetes ekstrak daun pacar air (Impatiens balsamina L.) terhadap ginjal tikus Wistar (Rattus norvegicus L.) yang diinduksi aloksan. Jurnal Biologi Pendidikan dan Terapan, 11(1), 110–120.
Nopiana, M., Putri, E. M., Novariyan, A., Pahrudin, I., Junaedi, C., & Rudiana, T. (2022). Karakterisasi fraksi aktif antioksidan dari biji gandaria (Bouea macrophylla). Alchemy: Journal of Chemistry, 11(1).
Ogbuehi, I., Adikwu, E., & Oputiri, D. (2014). Lipid lowering and appetite suppressive effect of leaves of Moringa oleifera Lam. in rats. British Journal of Pharmacology and Toxicology, 5(3), 103–108.
Pertiwi, W. A., et al. (2014). Pengaruh pemberian jus buah naga merah (Hylocereus polyrhizus) terhadap kadar HDL pria dislipidemia (pp. 12–13).
Rajan, N. S., & Bhat, R. (2016). Antioxidant compounds and antioxidant activities in unripe and ripe kundang fruits (Bouea macrophylla Griffith). Fruits, 71(1), 41–47.
Rosiana, I. W., & Widhiantara, I. G. (2020). Histologi lumen dan endotelium arteri dorsal penis tikus Wistar (Rattus norvegicus) yang diinduksi pakan tinggi lemak. Journal of Biological Sciences, 7(1), 73–79.
Sumarni, S., Anonim, T., & Supriyo, S. (2023). Gambaran Profil Lipid (Hdl, Ldl, Kolesterol Dan Trigliserid) Pada Orang Dengan Status Gizi Berlebih. Jurnal Lintas Keperawatan, 4(2): 312–317.
Teschke, R., Danan, G. (2018). Drug induced liver injury with analysis of alternative causes as confounding variables. Journal Clin Pharmacol. 84(7):1467-1477.
Vishnoi, H., Bodla, R., Kant, R., & Bodla, R. B. (2018). Green tea (Camellia sinensis) and its antioxidant property: A review. International Journal of Pharmaceutical Sciences and Research, 9(5), 1723–1736.
Wicaksono, S. (2019). Pengaruh pemberian spirulina peroral yang diberi CCl4 terhadap kadar ureum dan kreatinin darah tikus putih. Jurnal Kesehatan, 12(2), 86.
Weber, L.W., Meinrad, B., & Andreas, S. (2003) Hepatotoxicity and Mechanism of Action of Haloalkanes: Carbon Tetrachloride as a Toxicological Model. Journal of Toxicology. 33(2):105–136.
Wang, Y., et al. (2016). Hepatoprotective effects of anthocyanins from bilberry on CCl₄-induced liver injury in mice. Food and Chemical Toxicology, 91, 52–59.
Zaharul, M. I., Husnarika, F., & Syukriah. (2022). Efek pemberian ekstrak etanol rimpang jeringau terhadap gambaran morfohistologi ginjal tikus pasca induksi natrium nitrit. Jurnal Klorofil, 6(2), 1–13.
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