Calcium Assay of Tuna Bone Waste with Atomic Absorption Spectrophotometer (AAS)

Authors

DOI:

https://doi.org/10.33394/hjkk.v13i1.14705

Keywords:

waste, bone, tuna, Averrhoa bilimbi L., calsium assay

Abstract

Yellowfin tuna in Gorontalo is very abundant and is often exported outside the region and even abroad as fillets, so yellowfin tuna bones are also very plentiful. The community frequently processes yellowfin tuna bones into kuah asam or namely ilahe. One of the sources of ilahe is Averrhoa bilimbi L., but the leftover tuna bones are only a source of pollution. This research focuses on processing the waste into a product of high nutritional value and highlights the novelty of a more environmentally friendly fish bone calcium extraction method. One of the main novelties of this research is the use of natural materials, namely star fruit, as an alternative for calcium extraction, which reduces dependence on harmful chemicals and supports the principle of sustainability. The extraction method used heat, followed by drying to reduce moisture content, pulverizing (blender) to reduce particle size and sieving. The calcium content in tuna bone meal was then analyzed using the Atomic Absorption Spectroscopy (SSA) method. The results showed that the tuna bone meal was white in colour, and the calcium content was 184.4890 mg/g or 18.45%. This product has the potential to be applied in various fields, such as food, health, animal feed, pellets, and organic fertilizer, as well as a solution to reduce fishery waste and support environmental sustainability.

Author Biographies

Najmah Najmah, Universitas Negeri Gorontalo

Chemistry

Andi Indra Wulan Sari Ramadhani, Universitas Negeri Gorontalo

Physics

Devi Bunga Pagalla, Universitas Negeri Gorontalo

Biology

Arviani Arviani, Universitas Negeri Gorontalo

Chemistry

Vivi Dia Afrianti Sangkota, Universitas Negeri Gorontalo

Chemistry

References

Astiana, I., Nurjanah, N., & Nurhayati, T. (2016). Characterization of Acid Soluble Collagen from Redbelly Yellowtail Fusilier Fish Skin (Caesio cuning). Jurnal Pengolahan Hasil Perikanan Indonesia, 19(1), 79–93. https://doi.org/10.17844/jphpi.2016.19.1.79

Baba, M. , H. U. K. , & B. J. T. U. (2021). Effect of Fortification of Tuna Fish Bone Flour with Different Concentrations on Calcium Content and Organoleptic Quality of Tuna Fish Porridge Canned. Jurnal Agribisnis Perikanan, 14(2), 592–598.

Badan Pusat Statistik Provinsi Gorontalo. (2024). Juni 2024, nilai ekspor Provinsi Gorontalo sebesar US$3.891.147 dan nilai impor sebesar US$2.961.369. In Badan Pusat Statistik Provinsi Gorontalo.

Badan Standarisasi Nasional. (2005). Uji Kadar Kalsium (Ca) dengan Spektrofotometri Serapan Atom (SSA).

Cui, Y., Yang, L., Lu, W., Yang, H., Zhang, Y., Zhou, X., Ma, Y., Feng, J., & Shen, Q. (2021). Effect of Steam Explosion Pretreatment on the Production of Microscale Tuna Bone Power by Ultra-Speed Pulverization. Food Chemistry, 347.

Daeng, R. A. (2019). Pemanfaatan Tepung Tulang Ikan Cakalang (Katsuwonus pelamis) sebagai Sumber Kalsium dan Fosfor untuk Meningkatkan Nilai Gizi Biskuit. JURNAL BIOSAINSTEK, 1(01), 22–30. https://doi.org/10.52046/biosainstek.v1i01.209

Dewi, L. K., Supriadi, & Aminah, S. (2021). Analysis of Calcium (Ca) Levels in Milkfish’s (Chanos chanos) Bone Using Atomic Absorption Spectrophotometry (AAS). Jurnal Akademika Kimia, 10(1), 15–19.

Fitrasyah, S., Ma’rifat, M., Rahman, N., Nadila, D., Randani, A., & Ariani, A. (2023). Kadar zat gizi, daya antioksidan, dan organoleptik biskuit berbasis daun kelor dan tulang ikan tuna. Ghidza: Jurnal Gizi Dan Kesehatan, 7(2), 273–288.

Food And Agriculture Organization. (2021). Globefish Highlights 3rd issue 2021.

Haniastuti, T., Susilowati, H., & Rinastiti, M. (2020). Viability and alkaline phosphatase activity of human dental pulp cells after exposure to yellowfin tuna bone-derived hydroxyapatite in vitro. International Journal of Dentistry. https://doi.org/10.1155/2020/8857534

Hardiansyah, & Supariasa. (2016). Ilmu Gizi Teori & Aplikasi. EGC.

Hariyanto, Y. A., & Antasionasti, I. (2023). Penyuluhan Dan Pelatihan Pembuatan Pasta Gigi Herbal Dari Tulang Ikan Tuna. (Jurnal Masyarakat Mandiri, 7(5), 4753–4761. https://doi.org/N 2614-5758 | p-ISSN 2598-8158 : https://doi.org/10.31764/jmm.v7i5.17223

Hatta, M., Idayanti, R., & Hidayah, N. (2022). Organoleptik Paha Itik Magelang Dengan Perendaman Sari Belimbing Wuluh (averrhoa bilimbi l.) pada Konsentrasi Berbeda. Jurnal Ilmu Dan Teknologi Peternakan, 10(2), 46–51.

Hernawan, A. D., Anggresani, L., & Meirista, I. (2021). Formulasi pasta gigi hidroksiapatit dari limbah tulang ikan tenggiri (Scomberomorus guttatus). Chempublish Journal, 6(1), 34–45.

Husna, A., Handayani, A., & Syahputra, F. (2020). Pemanfaatan Tulang Ikan Kambing-Kambing (Abalistes stellaris) sebagai Sumber Kalsium Pada Produk Tepung Tulang Ikan. Acta Aquatica: Aquatic Sciences Journal, 7(1), 13–20.

Istiqlal, S. (2017). Proximate Levels of Bone Bluefin Tuna Fish As Gelatinization By Product. IOSR Journal of Environmental Science, Toxicology and Food Technology, 11(4), 12–17.

Kementerian Kelautan dan Perikanan. (2020). Laporan Tahunan Kementerian Kelautan dan Perikanan Tahun 2020.

Laitupa, I. W., & Husen, A. (2021). Effect Fortification of Tuna Fish Bone Flour and Anchovy Powder with Different Concentrations on the Calcium value of Ikan Tuna Kering Kayu Canned. Agrikan: Jurnal Agribisnis Perikanan, 13(2), 509–512.

Muhali, M., Hendrawani, H., Mirawati, B., & Hulyadi, H. (2023). Effectiveness of Activated Carbon CaCl2 and NaNO3 Reducing Fatty Acids and Increasing the Quantity of Biodiesel Production. Jurnal Penelitian Pendidikan IPA, 9(1), Article 1. https://doi.org/10.29303/jppipa.v9i1.2624

Musdalifah, S., Syamsidar, H. S., & Suriani, S. (2016). Dekolagenasi Limbah Tulang Paha Ayam Broiler (Gallus domesticus) oleh Natrium Hidroksida (NaOH) untuk Penentuan Kadar Kalsium (Ca) dan Fosfat (PO4). Al-Kimia, 4(2), 172–184. https://doi.org/10.24252/al-kimia.v4i2.1682

Muzaifa, M. (2018). Perubahan Komponen Kimia Belimbing Wuluh (Averrhoa bilimbi L.) Selama Pembuatan Asam Sunti. Jurnal Teknologi Pertanian Andalas, 22(1), 37. https://doi.org/10.25077/jtpa.22.1.37-43.2018

Nemati, M., Huda, N., & Ariffin, F. (2017). Development of calcium supplement from fish bone wastes of yellowfin tuna (Thunnus albacares) and characterization of nutritional quality. International Food Research Journal, 24(6), 2419–2426.

Novianty, H., R.A, S., & Jasmadi, J. (2024). Analyzing the Characteristics of Fishbone Powder Derived from Pangasius sp., Thunnus tonggol, and Thunnus albacares as Food Fortificant. agriTECH, 44(1), 90–100.

Raya, B. A., Kurniawan, H., & Nugraha, F. (2023). Karakterisasi Bobot Jenis dan Identifikasi Kalsium Pada Susu Kedelai. Journal Syifa Sciences and Clinical Research, 5(1), 37–43.

Savlak, N., Çağındı, Ö., Erk, G., Öktem, B., & Köse, E. (2020). Treatment Method Affects Color, Chemical, And Mineral Composition of Seabream (Sparus aurata) Fish Bone Powder From By-Products Of Fish Fillet. Journal of Aquatic Food Product Technology, 29(6), 592–602.

Sri, M., R, D., & Legowo, A. M. (2021). Karakteristik Kalsium Dari Tulang Ikan Bandeng (Chanos chanos) Yang Diekstraksi Menggunakan Larutan HCl. Journal of Nutrition College, 10(4), 321–327.

Sumarto, Desmelati, Sari, N. I., Angraini, R., & Arieska, L. (2021). Characteristic of Nano-Calcium Bone from a Different Species of Catfish (Pangasius hypophthalmus, Clarias batrachus, Hemibagrus nemurus and Paraplotosus albilabris). IOP Conference Series: Earth and Environmental Science, 695(1), 0–8.

Syazili, A., Ahmad, K., & Umakaapa, I. (2021). Using tuna fish bone waste as mineral sources in feed formulation of tilapia (Oreochromis niloticus). 2nd International Conference on Fisheries and Marine, 1–6. https://doi.org/10.1088/1755-1315/890/1/012026

Talib, A., & Noh, M. (2024). The Effects Of Additional Tuna Madihang Bone Fish Treatment On The Growth Of Catfish (clarias gariepinus) In Biofloc Pond In Ternate City North Maluku, Indonesia. Asian Food Science Journal, 23(11), 36–43.

Umam, K., Scabra, A. R., & Rahmmadani, T. B. C. (2024). Effect of Adding Tuna Fish Bone Meal as a Source of Calcium Minerals in Feed in Freshwater Vannamei Shrimp (Litopenaeus vannamei) Cultivation. Jurnal Akuakultura, 8(2), 44–50.

Wijayanti, I., Rianingsih, L., & Amalia, U. (2018). Karakteristik Fisikokimia Kalsium Dari Tulang Nila (Oreochromis niloticus) Dengan Perendaman Belimbing Wuluh. Jurnal Pengolahan Hasil Perikanan Indonesia., 21(2), 336-344.

Yuliani, Y., Marwati, M., Wardana, H., Emmawati, A., & Candra, K. (2018). Karakteristik Kerupuk Ikan Dengan Substitusi Tepung Tulang Ikan Gabus (Channa striata) Sebagai Fortifikan Kalsium. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(2), 259. https://doi.org/10.17844/jphpi.v21i2.23042

Downloads

Additional Files

Published

2025-02-28

How to Cite

Najmah, N., Ramadhani, A. I. W. S., Pagalla, D. B., Arviani, A., & Sangkota, V. D. A. (2025). Calcium Assay of Tuna Bone Waste with Atomic Absorption Spectrophotometer (AAS). Hydrogen: Jurnal Kependidikan Kimia, 13(1), 49–57. https://doi.org/10.33394/hjkk.v13i1.14705

Issue

Vol. 13 No. 1 (2025): February 2025

Section

Articles

Citation Check

License

License and Publishing Agreement
In submitting the manuscript to the journal, the authors certify that:

  1. They are authorized by their co-authors to enter into these arrangements.
  2. The work described has not been formally published before, except in the form of an abstract or as part of a published lecture, review, thesis, or overlay journal.
  3. That it is not under consideration for publication elsewhere,
  4. That its publication has been approved by all the author(s) and by the responsible authorities – tacitly or explicitly – of the institutes where the work has been carried out.
  5. They secure the right to reproduce any material that has already been published or copyrighted elsewhere.
  6. They agree to the following license and publishing agreement.

Copyright
Authors who publish with Hydrogen: Jurnal Kependidikan Kimia agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal. 
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Licensing for Data Publication

Hydrogen: Jurnal Kependidikan Kimia uses a variety of waivers and licenses, that are specifically designed for and appropriate for the treatment of data: Open Data Commons Attribution License, http://www.opendatacommons.org/licenses/by/1.0/ (default) Other data publishing licenses may be allowed as exceptions (subject to approval by the editor on a case-by-case basis) and should be justified with a written statement from the author, which will be published with the article.