Alpha Amylase Inhibitory Activity of Curcumin Analogs and Its Synergy with Ferulic Acid in Vitro

Authors

  • Khoirotun Nafillah Politeknik Maritim Negeri indonesia, Indonesia http://orcid.org/0009-0001-0235-7287
  • Helena Devi Ariyani Politeknik Maritim Negeri Indonesia, Indonesia
  • Lia Retian Asfirah Politeknik Maritim Negeri Indonesia, Indonesia

DOI:

https://doi.org/10.33394/hjkk.v13i3.15683

Keywords:

α-amylase, curcumin analogs, synergistic, ferulic acid

Abstract

Curcumin analog compounds are α,β unsaturated compounds through simplifying the beta diketo group to monoketo, which has better bioavailability and a more stable structure than curcumin compounds. This study aims to determine the α-amylase inhibitory activity on symmetric curcumin analog compounds, namely the compounds 2,6-bis(3,4-dimethoxybenzylidine)cyclohexanone (A) and 2,6-bis(3,4-dimethoxybenzylidine)cyclopentanone (B). as well as testing its synergistic interaction with ferulic acid in vitro. The α-amylase inhibition test was carried out using an iodine reagent and a starch solution as a substrate. The absorbance value was measured using a UV-vis spectrophotometer (λ 568 nm), and the % inhibition was calculated. The average value of the optimum α-amylase inhibition percentage for compounds A, B, and ferulic acid, respectively is 58.17%; 22.95%, and 93.52%. Based on the synergistic interaction, it was concluded that compounds A and B showed synergistic activity with ferulic acid. The percentage of α-amylase inhibition in the concentration ratio of curcumin analog A: ferulic acid (1:8) was 98.65%, and curcumin analog B: ferulic acid (1:4) was 98.37%. This shows that combining compounds between symmetrical curcumin analogs and ferulic acid can increase the activity of antidiabetic drug candidate compounds compared to single compounds. This study offers a new approach by testing the potential combination of curcumin and ferulic acid analogues as α-amylase inhibitors in vitro, demonstrating a synergy that has not been widely explored and opening up opportunities for developing more effective natural antidiabetic therapies.

Author Biographies

Khoirotun Nafillah, Politeknik Maritim Negeri indonesia

Jurusan Teknika

Helena Devi Ariyani, Politeknik Maritim Negeri Indonesia

Jurusan Teknika

Lia Retian Asfirah, Politeknik Maritim Negeri Indonesia

Jurusan Bisnis Maritim

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Published

2025-06-30

How to Cite

Nafillah, K., Ariyani, H. D., & Asfirah, L. R. (2025). Alpha Amylase Inhibitory Activity of Curcumin Analogs and Its Synergy with Ferulic Acid in Vitro. Hydrogen: Jurnal Kependidikan Kimia, 13(3), 428–437. https://doi.org/10.33394/hjkk.v13i3.15683

Issue

Vol. 13 No. 3 (2025): June 2025

Section

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